Accurate & Flexible PCR Master Mix Calculations Made Easy

Setting up multiple PCR reactions requires careful calculation to ensure consistency and conserve precious reagents. Westlab's enhanced Flexible PCR Master Mix Calculator streamlines this crucial step, saving you time and reducing the potential for pipetting errors.
Simply define your reaction parameters – single reaction volume, number of reactions, and desired overage (percentage or extra reactions). Then, input the stock and final concentrations for your components using the provided table, which includes common reagents like Buffer, dNTPs, Primers, MgCl₂, and Polymerase, plus two rows for your own custom components.
Key Features:

• • Automatic Molar Unit Conversion: Effortlessly handles inputs across M, mM, µM, and nM – no more manual pre-calculation needed! (For other units like 'X' or 'U/µL', ensure stock and final units match).

  • Flexible Component Input: Use standard components and add your own specific reagents.

  • Overage Calculation: Easily add overage by percentage or number of reactions.

  • Clear Results Summary: Instantly see the required volume of water, the total master mix volume to prepare, and the volume of mix to aliquot per reaction tube.

  • Detailed Component List: Provides the calculated total volume needed for each individual component in the master mix.

  • Improved Validation: Checks for common errors like insufficient stock concentration (after unit conversion) and highlights problematic inputs directly in the table.

Spend less time with calculations and more time focusing on your results. This tool helps ensure your PCR setup is accurate, consistent, and efficient.