Nucleic Acid Concentration & Molar Converter

Effortlessly Convert Nucleic Acid Concentrations & Assess Purity

Stop juggling multiple formulas and calculators! Westlab's Nucleic Acid Concentration & Molar Converter provides a seamless way to switch between all common units used for quantifying DNA and RNA. Whether you start with an OD₂₆₀ reading, a mass concentration, or a molar value, this tool quickly calculates the equivalents.

Simply select your nucleic acid type (dsDNA, ssDNA, or RNA) and input your known value along with its unit (e.g., OD₂₆₀, ng/µL, µg/mL, µM, pmol/µL, copies/µL). The calculator instantly displays the corresponding values in all other standard units.

Key Features:

  • Handles dsDNA, ssDNA, and RNA: Uses standard, widely accepted conversion factors (e.g., 50/33/40 µg/mL/OD) and average molecular weights (e.g., ~650 g/mol/bp for dsDNA, ~330 g/mol/nt for ssDNA/RNA).
  • Length Dependent Calculations: Input the length (bp or nt) of your molecule to enable accurate molar concentration (µM, pmol/µL, nM), copy number (copies/µL), and ng/pmol conversions. Results requiring length will be indicated if it's missing.
  • Optional Purity Check: Enter your A280 reading alongside the A260 value (if OD is your input) to calculate the A260/A280 ratio, providing a quick indication of sample purity.
  • OD Range Warning: Flags OD₂₆₀ values outside the typical reliable spectrophotometer range.
  • Transparency: Notes clearly state the standard factors and average MWs used in the calculations.

Ideal for molecular biologists, biochemists, and anyone working with nucleic acid quantification, this tool saves time and reduces calculation errors when preparing samples for PCR, sequencing, cloning, and other applications.

Nucleic Acid Concentration & Purity Converter

Nucleic Acid Concentration & Purity Converter

Convert between OD₂₆₀, mass (ng/µL, µg/mL, µg/µL), molarity (µM, pmol/µL, nM), and copy number for dsDNA, ssDNA, or RNA. Optionally calculates A260/A280 ratio.

Input Parameters


Calculated Equivalent Concentrations & Purity

OD₂₆₀ (10mm path):-
Mass Conc:- ng/µL
Mass Conc:- µg/mL
Mass Conc:- µg/µL
Molar Conc:- µM
Molar Conc:- pmol/µL
Molar Conc:- nM
Copy Number:- copies/µL
Mass / Mole:- ng/pmol
A260 / A280 Ratio:-

Factors used: OD₁₀mm ≈ 50 µg/mL (dsDNA), 33 µg/mL (ssDNA), 40 µg/mL (RNA). Avg MW ≈ 650 g/mol/bp (dsDNA), 330 g/mol/nt (ssDNA/RNA). Molar, Copy Number, and ng/pmol calculations require molecule Length (>0). Purity ratio requires A280 input.

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