Accurately determining cell concentration and viability is crucial for successful cell culture experiments, from routine passaging to complex assays. Westlab's Cell Viability & Concentration Calculator simplifies this essential task, utilizing standard hemocytometer counts obtained via the Trypan Blue exclusion method.

Simply input your viable (unstained) and dead (blue-stained) cell counts, the number of large squares counted on your hemocytometer grid, and the dilution factor used during staining. The calculator instantly provides:

• • Viable Cell Concentration

  • Total Cell Concentration

  • Percentage Viability (with helpful interpretation feedback: Excellent, Good, Fair, Poor)

Enhanced Features:

• Display concentrations in your preferred units (cells/mL, x10⁵ cells/mL, or x10⁶ cells/mL).

• Optionally calculate the total number of viable and all cells in your original suspension by entering its volume.

• Advanced option to use a custom hemocytometer factor if using non-standard chambers.

Based on standard counting principles, this tool reduces manual calculation errors and provides reliable data for consistent cell culture management and downstream applications like cell seeding (use these results in our Seeding & Passaging Calculator!).