BENTO FIREPol® Master Mix Ready To Load, 400µL (100 x 20µL PCR rxns)

5x FIREPol® Master Mix Ready To Load is a highly optimised PCR master mix that simplifies and accelerates PCR setup for routine applications. This ready-to-use formulation contains all essential components required for PCR amplification, including FIREPol® DNA polymerase, reaction buffer, MgCl₂, dNTPs, and tracking dyes for easy loading onto agarose gels. This master mix offers convenience and reliability for routine PCR assays.

Convenience & Time-Saving

Designed to streamline the PCR process, 5x FIREPol® Master Mix Ready To Load eliminates the need for additional reagents such as loading buffer. It enables direct loading of PCR products onto an agarose gel, saving time and reducing the risk of errors associated with additional preparation steps. The inclusion of tracking dyes allows for easy visualisation during electrophoresis.

Robust Performance

The master mix delivers high yield and reproducibility across a wide range of DNA templates and amplicon sizes. Its robust enzymatic activity is ideal for standard PCR applications, providing consistent, high-quality results, even with low-copy targets or complex DNA mixtures. Whether you're amplifying small or large DNA fragments, this mix ensures reliable amplification.

Storage & Stability

To maintain optimal performance, store 5x FIREPol® Master Mix Ready To Load at –20ºC for long-term storage. Once thawed, the mix can be stored at 2–8ºC for up to six months or room temperature for up to one month, with no significant loss in quality. Avoid repeated freeze-thaw cycles to preserve reagent integrity and performance.

Applications

This master mix is perfect for a variety of molecular biology applications, including DNA cloning, genotyping, screening, and sequencing workflows. It is especially suitable for laboratories looking for a fast, reliable PCR setup with minimal preparation and handling steps. The mix is compatible with standard PCR instruments, making it an easy-to-use solution for routine PCR assays.

Product Features

• Ready-to-Load Format: Enables direct gel electrophoresis post-PCR, reducing preparation time and eliminating the need for separate loading buffer.
• Optimised 5× Concentration: Ensures reliable amplification with minimal preparation and high robustness.
• High Reproducibility: Delivers consistent results across various sample types and experimental conditions.
• Integrated Tracking Dye: Allows easy sample visualisation and direct loading onto an agarose gel.
• All-in-One Formulation: Includes all necessary components—DNA polymerase, buffer, MgCl₂, dNTPs, and tracking dye - requiring no additional reagents.
• Cold Storage Compatible: Stable at 4ºC for up to one month once thawed, offering flexibility in storage.
• Versatile Use: Ideal for a broad range of PCR applications, from routine assays to more complex workflows.