BENTO FIREPol® Master Mix Ready To Load, 100µL (25 x 20µL PCR rxns)
FIREPol® Master Mix Ready to Load is a 5× concentrated PCR mixture developed to simplify and accelerate PCR setup. It is an optimised, pre-mixed formulation containing all essential components for successful DNA amplification - including FIREPol® DNA Polymerase, reaction buffer, MgCl₂, dNTPs, and a tracking dye for direct loading onto agarose gels.
Convenience & Time-Saving
Designed for ease of use, this master mix reduces hands-on preparation time and minimises the risk of contamination or pipetting errors. The inclusion of gel tracking dyes eliminates the need to add loading buffer post-PCR, enabling samples to be loaded directly onto an agarose gel for electrophoresis.
Robust Performance
The formulation ensures high yield and reproducibility across various DNA templates and amplicon sizes. Its robust enzymatic activity is suited for standard and routine PCR applications, delivering consistent results even with low-copy targets or complex DNA mixtures.
Storage & Stability
FIREPol® Master Mix Ready to Load should be stored at –20 °C for long-term use. Thawed mixes can be stored at 4°C for up to one month without significant performance loss. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Applications
Ideal for general molecular biology applications, including DNA cloning, genotyping, screening, and sequencing workflows. The mix is especially suitable for laboratories seeking a fast, reliable PCR setup with minimal additional steps.
Product Features
- Ready-to-Load Format: Enables direct gel electrophoresis post-PCR, saving time and reducing errors.
- Optimised 5× Concentration: Ensures robust amplification with minimal preparation.
- High Reproducibility: Supports consistent results across various sample types and conditions.
- Integrated Tracking Dye: Eliminates need for separate gel loading buffer.
- All-in-One Formulation: Includes buffer, dNTPs, MgCl₂, and enzyme - no additional components required.
- Cold Storage Compatible: Maintains stability at 4 °C for up to one month once thawed.
- Versatile Use: Suitable for a broad range of standard PCR applications.
Specification
| Label | Value |
|---|---|
| Brand | BENTO |
| Range | FIREPol® |
| Type | 5× Concentrated Master Mix, Ready To Load |
| Reaction Volume | 100µL (Sufficient for 25 x 20µL PCR Reactions) |
| DNA Polymerase | FIREPol® DNA Polymerase |
| dNTP Concentration (Final) | 0.2 mM Each (In 1x Reaction) |
| MgCl₂ Concentration (Final) | 1.5 mM (In 1x Reaction) |
| Loading Dye | Included, it allows direct gel loading post-PCR. |
| Buffer Components | Optimised Buffer, dNTPs, MgCl₂, & Tracking Dye |
| Storage Temperature | –20 °C (Long-Term); 4 °C (Short-Term, ≤1 Month) |
| Stability After Thawing | Stable for up to 1 month at 4 °C. |
| Application Suitability | Standard PCR, genotyping, screening, and DNA amplification. |
Product Questions
Estimated Lead Times
Special Order products are ordered from the manufacturer once your order is placed.
Please allow up to 6 weeks for processing and delivery.
Specification
| Label | Value |
|---|---|
| Brand | BENTO |
| Range | FIREPol® |
| Type | 5× Concentrated Master Mix, Ready To Load |
| Reaction Volume | 100µL (Sufficient for 25 x 20µL PCR Reactions) |
| DNA Polymerase | FIREPol® DNA Polymerase |
| dNTP Concentration (Final) | 0.2 mM Each (In 1x Reaction) |
| MgCl₂ Concentration (Final) | 1.5 mM (In 1x Reaction) |
| Loading Dye | Included, it allows direct gel loading post-PCR. |
| Buffer Components | Optimised Buffer, dNTPs, MgCl₂, & Tracking Dye |
| Storage Temperature | –20 °C (Long-Term); 4 °C (Short-Term, ≤1 Month) |
| Stability After Thawing | Stable for up to 1 month at 4 °C. |
| Application Suitability | Standard PCR, genotyping, screening, and DNA amplification. |
Product Questions
Estimated Lead Times
Special Order products are ordered from the manufacturer once your order is placed.
Please allow up to 6 weeks for processing and delivery.
