Master Your Cell Culture Workflow: Seeding & Passaging Calculator

Achieve consistent, reproducible cell cultures with Westlab's Cell Culture Seeding & Passaging Assistant. This essential tool simplifies the routine calculations needed for maintaining healthy cell lines and setting up experiments accurately.

Whether you need to plate cells at a specific density or passage your cultures, this calculator handles the math:

• Seeding Mode: Calculate the exact volume of cell suspension required to seed new flasks, dishes, or multi-well plates based on your target density (either cells/cm² for adherent cultures or cells/mL for suspension cultures).
• Passaging Mode: Plan your cell splitting accurately. Calculate the required volumes based on a desired split ratio (e.g., 1:5, 1:10), or determine the volumes needed to achieve a specific target seeding density in the new vessels.

The tool includes presets for common culture vessel formats (T-flasks, plates, dishes) to automatically use standard surface areas and typical volumes, or you can input custom parameters. Optionally, input your cell line's estimated doubling time and target confluency details to get a rough estimate of when your newly seeded cultures might be ready for the next step.

Eliminate guesswork, ensure consistent seeding densities, optimize your passaging strategy, and streamline your cell culture workflow with this handy calculator.