An Essential Guide to Using a Volumetric Pipette

Accuracy and precision stand at the forefront of any scientific endeavor, and in the world of lab work, a Volumetric Pipette is a critical tool that delivers on both fronts. Designed to draw and dispense a fixed volume of liquid with great accuracy, pipettes play an indispensable role in any laboratory setting. This guide serves to illuminate the process of using a pipette correctly and effectively.

What is a Volumetric Pipette?

A Volumetric Pipette is a laboratory tool designed to measure and transfer a specific, fixed volume of liquid. It is known for providing highly accurate and precise measurements in scientific experiments.

These pipettes, unlike a pipette dropper, are designed for precision. It's a long tube calibrated to hold a specific volume of liquid with remarkable accuracy. An important feature of the Volumetric Pipette is its single calibration mark that denotes the volume it can hold. These pipettes are one of the most accurate tools available to a scientist when it comes to measuring liquids.

How to Use a Volumetric Pipette?

Using a volumetric pipette requires care and understanding. Here is a step-by-step guide to use it properly:

• Cleaning: Always start with a clean pipette to ensure no residual substances affect your measurements.
• Filling: Attach the pipette filler to the top of the pipette. The filler safely draws the liquid up to the calibration mark.
• Reading: Ensure the base of the liquid's meniscus (curve) aligns with the calibration mark. To read it accurately, place your eyes level with the mark and avoid parallax errors.
• Dispensing: Carefully release the liquid into the receiving vessel. Be sure not to blow out the remaining liquid in the tip, as this is accounted for in the calibration.

Different Types of Pipettes

Pipette comes in various types, each serving unique functions and needs in a laboratory setting. Here are some of the common types:

• Volumetric Pipettes: These pipettes are designed to measure a specific volume of liquid with high precision. They are often used in analytical chemistry to prepare solutions.
• Graduated Pipettes: Also known as Mohr pipettes, they have graduations along the tube, allowing for different volumes of liquid to be measured and dispensed.
• Pasteur Pipettes: Named after Louis Pasteur, they are similar to droppers and made of glass or plastic. They are used for less precise measurements and the transfer of liquids.
• Micropipettes: These are used for extremely small volumes, typically in the microliter range. They are essential in molecular biology laboratories.
• Pipette Fillers: This isn't a type of pipette but rather an accessory used to draw liquid into a pipette. They are especially useful when dealing with large pipettes or dangerous liquids.

It's important to choose the right type of dropper or pipette based on your specific needs to ensure accuracy and safety in your scientific experiments.

How to Maintain and Care for Your Equipment?

Good practice calls for maintaining your equipment to ensure its longevity and performance. Always rinse your pipette with distilled water after use. If you have used the pipette for strong acids or bases, be sure to rinse it with a suitable solvent first and then with distilled water.

When not in use, store your pipette in a Pipette Stand. This will keep the pipette safe and prevent potential cross-contamination.

With the right knowledge, tools, and practices, the pipette can provide unmatched precision in the lab. Always remember the importance of using a pipette filler for safety, a Pipette Stand for storage, and the significance of sourcing your equipment from reliable Scientific Equipment Suppliers. Master the use of the pipette, and you're well on your way to achieving high levels of accuracy and precision in your scientific work.

The Importance of Quality Equipment | Get the Right Supplier

While knowing how to use a pipette is crucial, using quality equipment is equally important. You should always make sure your scientific tools are sourced from reputable laboratory equipment suppliers. 

A trusted supplier guarantees you reliable, high-performance equipment that's crucial for maintaining accuracy in your experiments. Your equipment supplier should also provide necessary accessories such as Pipette Stands and pipette fillers. A stand is important for safe storage and to avoid contamination or damage to your pipettes.

It is recommended to take the time to research different suppliers, compare their offerings, and read reviews from other customers. However, if you’re looking for high quality on a budget, Westlab Australia is here to provide you with just that. 

Visit our website for more information and order your laboratory equipment today!

FAQs

What is a pipette dropper?

A Pipette Dropper is a laboratory apparatus used to accurately measure and transport specific volumes of liquid.

What is a graduated pipette?

A graduated pipette is a type of laboratory tool used to measure and transfer varying volumes of liquid. Unlike a Volumetric Pipette, it has multiple markings along its length, allowing for a range of volumes to be measured.

How can I find reputable scientific equipment suppliers?

To find reputable lab equipment suppliers, conduct thorough research, compare offerings, read customer reviews, and ask for recommendations from colleagues.